rabbit anti mlph (Proteintech)

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Proteintech rabbit anti mlph
Rabbit Anti Mlph, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mlph+antibody/RNF4+Antibody/pmc12959181-110-20-23
Average 93 stars, based on 14 article reviews

rabbit anti mlph - by Bioz Stars, 2026-10

93/100 stars

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Article Title: P300 regulates Melanophilin expression by modulating TFAP2A binding through histone acetylation.
Article Snippet: Background: Melanophilin is an effector protein that interacts with Rab27a and Myosin Va and regulates melanosome transport in melanocytes.. Type 3 Griscelli syndrome, a mutation in Mlph gene, is characterized by partial pigment dilution, without any associated systemic problems.. P300 plays roles in histone acetylation and changes chromatin state.

Article Title: MLPH regulates EMT in pancreatic adenocarcinoma through the PI3K-AKT signaling pathway
Article Snippet: Proteintech: MLPH, 1:1000; Vimentin, 1:1000; β-actin, 1:10000) overnight at 4°C.

Article Title: Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression
Article Snippet: Β-actin antibody (1:10,000, GeneTex, GTX629630), Myosin Va antibody (1:800, Cell Signaling Technology, Beverly, MA, 3402S), Rab27a antibody (1:500, Santa Cruz, CA, sc-74586), Mlph antibody (1:600, Protein Tech Group, Inc, Chicago, IL, 10338-1-AP), DNMT1 antibody (1:1,000, Cell Signaling Technology, Beverly, MA, 5032), Sp1 antibody (1:2,000, Protein Tech Group, Inc, Chicago, IL, 21962–1-AP), and HDAC5 antibody (1:500, Protein Tech Group, Inc, Chicago, IL, 16166-1-AP) were used.

Article Title: Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression.
Article Snippet: Β-actin antibody (1:10,000, GeneTex, GTX629630), Myosin Va antibody (1:800, Cell Signaling Technology, Beverly, MA, 3402S), Rab27a antibody (1:500, Santa Cruz, CA, sc-74586), Mlph antibody (1:600, Protein Tech Group, Inc, Chicago, IL, 10338-1-AP), DNMT1 antibody (1:1,000, Cell Signaling Technology, Beverly, MA, 5032), Sp1 antibody (1:2,000, Protein Tech Group, Inc, Chicago, IL, 21962–1-AP), and HDAC5 antibody (1:500, Protein Tech Group, Inc, Chicago, IL, 16166-1-AP) were used.

Incubation:

Article Title: 16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression.
Article Snippet: Background: Rab27a, Mlph, and MyoVa form a tripartite complex and relate to melanosome distribution.. Melanophilin (Mlph) acts as a linker protein between Rab27a and MyoVa.. The biological activity and function of 16-kauren on the expression of Mlph has not yet been studied.

Article Title: Inhibition of Melanosome Transport by Inducing Exon Skipping in Melanophilin
Article Snippet: .. Blots were incubated overnight with Mlph antibody (1:500, Protein Tech Group, Inc, Chicago, IL, USA), or β-actin antibody (1:5,000, Sigma-Aldrich) at 4°C. .. Blots were then rinsed three times with 1X TBS-T and incubated with horseradish peroxidase-conjugated anti-rabbit (Bethyl Laboratories, Montgomery, AL, USA) or anti-mouse (Bio-Rad, Hercules, CA, USA) antiserum at room temperature for 1 h. Bound antibodies were detected using a WEST-ZOL ® Plus Western Blot Detection System (INtRON Biotechnology, Seoul, Korea) and SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).

Article Title: Inhibition of Melanosome Transport by Inducing Exon Skipping in Melanophilin.
Article Snippet: .. Blots were incubated overnight with Mlph antibody (1:500, Protein Tech Group, Inc, Chicago, IL, USA), or -actin antibody (1:5,000, Sigma-Aldrich) at 4°C. .. Blots were then rinsed three times with 1X TBS-T and incubated with horseradish peroxidase-conjugated anti-rabbit (Bethyl Laboratories, Montgomery, AL, USA) or anti-mouse (Bio-Rad, Hercules, CA, USA) antiserum at room temperature for 1 h. Bound antibodies were detected using a WEST-ZOL® Plus Western Blot Detection System (INtRON Biotechnology, Seoul, Korea) and SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).

Membrane:

Article Title: A novel function of Prohibitin on melanosome transport in melanocytes
Article Snippet: .. Equal amount of proteins was separated by NuPAGER 10% bis-tris gel (Invitrogen, CA, USA) and transferred onto a polyvinylidene fluoride transfer membrane. β-actin antibody (1:10,000, GeneTex, GTX629630), Myosin-Va antibody (1:800, Cell Signaling Technology, Beverly, MA, 3402S), Rab27a antibody (1:500, Santa Cruz, CA, sc-74586), Mlph antibody (1:600, Protein Tech Group, Inc, Chicago, IL, 10338-1-AP), MADD antibody (1:700, Thermo Scientific, USA, PA5-19879), PHB1 antibody (1:500, Invitrogen, CA, MA5-12858), PHB2 antibody (1:500, Invitrogen, PA1-29883), poly-histidine antibody (1:5,000, Sigma, St. Louis, MO, USA, H1029), FLAG antibody (1:1,000, Sigma, St. Louis, MO, USA, F1804) and T7 antibody (1:10,000, Novagen, Germany, 69522-3) were kept at 4 °C overnight. .. Horseradish peroxidase conjugated for anti-rabbit (1:80,000, Sigma, St. Louis, MO, USA, A0545) or anti-mouse (1:20,000, Sigma, St. Louis, MO, USA, A9044) were used at 4 °C for 2 h. Binding antibodies were detected using an SuperSignal TM West Pico PLUS Chemiluminescent substrate (Thermo Scientific, USA).

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